Journal: Oncogene

Article Title: Transduction of the SkBr3 breast carcinoma cell line with the HOXB7 gene induces bFGF expression, increases cell proliferation and reduces growth factor dependence.

doi: 10.1038/sj.onc.1201875

Figure Lengend Snippet: Figure 1 Expression of HOXB7 and bFGF genes in A375 melanoma, SkBr3 mammary carcinoma and HOXB7-transduced SkBr3. (a) RNase protection analysis. Riboprobes (R) and protected fragments (P) are shown on the right, and molecular size (base pairs) markers (MWM) (pGEM4Z HpaII) on the left. b-actin expression is shown as internal control. (b) Northern blot analysis. b2 microglobulin expression is shown as internal control. (c) Western blot analysis of bFGF produced by A375 melanoma, SkBr3 mammary carcinoma and HOXB7-transduced SkBr3 cells

Article Snippet: Intracellular and secreted bFGF were quanti®ed by a human bFGF ELISA from R&D systems (Minneapolis, MN).

Techniques: Expressing, Control, Northern Blot, Western Blot, Produced

Journal: Oncogene

Article Title: Transduction of the SkBr3 breast carcinoma cell line with the HOXB7 gene induces bFGF expression, increases cell proliferation and reduces growth factor dependence.

doi: 10.1038/sj.onc.1201875

Figure Lengend Snippet: Figure 3 (a) Role of bFGF for SkBr3/HOXB7 cell growth in low serum. Growth kinetics of parental and HOXB7-transduced SkBr3 cells maintained in 10% or 1% FCS were compared. Cell growth was monitored over the indicated time intervals by methylene blue inclusion, as indicated in Materials and methods. (b) Eect of exogenous rbFGF on SkBr3 cell growth. Growth curves of cells maintained in 1% FCS plus dierent concentra- tions of rbFGF evaluated by methylene blue inclusion

Article Snippet: Intracellular and secreted bFGF were quanti®ed by a human bFGF ELISA from R&D systems (Minneapolis, MN).

Techniques:

Journal: Oncogene

Article Title: Transduction of the SkBr3 breast carcinoma cell line with the HOXB7 gene induces bFGF expression, increases cell proliferation and reduces growth factor dependence.

doi: 10.1038/sj.onc.1201875

Figure Lengend Snippet: Figure 4 Inhibition of SkBr3/HOXB7 cell proliferation upon treatment with 30 mM of bFGF antisense (a) or of sense (s) oligomers. Evidence of bFGF intracrine loop operating in cells kept in low serum. Mean+s.d. of three separate experiments is shown

Article Snippet: Intracellular and secreted bFGF were quanti®ed by a human bFGF ELISA from R&D systems (Minneapolis, MN).

Techniques: Inhibition

Journal: The Journal of Biological Chemistry

Article Title: Reactivation of Mitogen-activated Protein Kinase (MAPK) Pathway by FGF Receptor 3 (FGFR3)/Ras Mediates Resistance to Vemurafenib in Human B-RAF V600E Mutant Melanoma *

doi: 10.1074/jbc.M112.377218

Figure Lengend Snippet: Enhanced FGFR3 activation in vemurafenib-resistant B-RAF V600E melanoma cells. A, phospho-RTK antibody array analysis. Cell lysates from A375, M14, A375-R1, and M14-R cell lines were incubated on RTK antibody array for 16 h and phosphorylation status was determined as described under “Experimental Procedures.” Each RTK antibody is spotted in duplicate. Supplemental Table S2 describes the list of RTKs and the layout of the antibody array. B, confirmation of phospho-FGFR3 levels by Western blot analysis. Protein levels of total and phosho-FGFR3 were assessed using immunoblotting. C, ELISA analysis of secreted FGF2 in the conditioned media obtained from A375, A375-R1, M14, and M14-R cells. ELISA was performed as described in “Experimental Procedures.”

Article Snippet: Cells were cultured for 48 h in growth medium described above, and the conditioned medium samples (cell free culture supernatant) were analyzed for concentrations of human FGF2 using human FGF2 Quantikine ELISA Kit (R&D Systems).

Techniques: Activation Assay, Ab Array, Incubation, Phospho-proteomics, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Materials & Design

Article Title: Multifunctional nanofiber-based dressings in coordination with adipose-derived stem cells for accelerated burn wound healing

doi: 10.1016/j.matdes.2025.113929

Figure Lengend Snippet: Fig. 4. (A) Detection of ADSCs proliferation by ADM coating at different times. Quantification of (B) EGF and (C) FGF2 in ADSCs paracrine products. *** P < 0.001 representing a significant difference as compared with ADM (3 min) and TLWDA.

Article Snippet: Human EGF enzyme-linked immunosorbent assay (ELISA) kit and human FGF2 ELISA kit were purchased from BOSTER (Wuhan, China).

Techniques:

Journal: Neuropsychiatric Disease and Treatment

Article Title: Synapse-Related Serum and P300 Biomarkers Predict the Occurrence of Mild Cognitive Impairment in Depression

doi: 10.2147/NDT.S448312

Figure Lengend Snippet: Comparison of baseline serum indices between DWM group and DWOM group. ( A ) The comparison of serum BDNF levels. ( B ) The comparison of serum FGF2 levels. ( C ) The comparison of serum FGF7 levels. ( D ) The comparison of serum FGF22 levels.

Article Snippet: The baseline serum levels of BDNF (Proteintech, USA, Cat No. KE00096), FGF2 (Proteintech, USA, Cat No. KE00129), FGF7 (Abcam, Britain, Cat No. ab314148) and FGF22 (CUSABIO, China, Cat No. CSB-EL008628HU) in patients were measured using ELISA kits according to the manufacturer’s instructions.

Techniques: Comparison

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

doi: 10.1590/1414-431X20132929

Figure Lengend Snippet: Morphology of bone marrow progenitor cells 48 h after infection with Ad-BMP2-bFGF-GFP at a multiplicity of infection (MOI) value of 5000. A , Image under light microscope. B , Image under fluorescence microscope showing the expression of green fluorescent protein-positive cells. Bar = 50 μm.

Article Snippet: The concentrations of BMP2 and bFGF in the supernatants were determined with a human BMP2 ELISA kit (Boster, China) and a human bFGF ELISA kit (Boster, China), according to the manufacturers' instructions.

Techniques: Infection, Light Microscopy, Fluorescence, Microscopy, Expressing

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

doi: 10.1590/1414-431X20132929

Figure Lengend Snippet: Infection efficiency of Ad-BMP2-bFGF in bone marrow progenitor cells. The infection efficiency was calculated by the number of green fluorescent protein-positive cells/total number of nuclei stained with DAPI x100 (%) (n=3). MOI: multiplicity of infection.

Article Snippet: The concentrations of BMP2 and bFGF in the supernatants were determined with a human BMP2 ELISA kit (Boster, China) and a human bFGF ELISA kit (Boster, China), according to the manufacturers' instructions.

Techniques: Infection, Staining

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

doi: 10.1590/1414-431X20132929

Figure Lengend Snippet: Expression of bone morphogenetic protein 2 (BMP-2) and basic fibroblast growth factor (bFGF) in bone marrow progenitor cells (BMPCs). A , ELISA assay of BMP2 level secreted by BMPCs at different days after infection. B , ELISA assay of bFGF level secreted by BMPCs at different days after infection. Data are reported as means±SD (n=3). *P<0.05 vs control (ANOVA).

Article Snippet: The concentrations of BMP2 and bFGF in the supernatants were determined with a human BMP2 ELISA kit (Boster, China) and a human bFGF ELISA kit (Boster, China), according to the manufacturers' instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Infection, Control

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

doi: 10.1590/1414-431X20132929

Figure Lengend Snippet: Alkaline phosphatase (ALP) activity in bone marrow progenitor cells (BMPCs). A , ALP activity detected in BMPCs at different days after infection. *P<0.05 vs control. # P<0.05 vs Ad-BMP2-infected BMPCs. B , ALP staining of BMPCs 21 days after infection with Ad-BMP2-bFGF. Many brown-black particles appeared in the cytoplasm, and cells were stained blue. Bar = 100 μm.

Article Snippet: The concentrations of BMP2 and bFGF in the supernatants were determined with a human BMP2 ELISA kit (Boster, China) and a human bFGF ELISA kit (Boster, China), according to the manufacturers' instructions.

Techniques: Activity Assay, Infection, Control, Staining

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

doi: 10.1590/1414-431X20132929

Figure Lengend Snippet: Kossa staining of bone marrow progenitor cells 21 days after infection with Ad-BMP2-bFGF or Ad-BMP2. Mineralized calcium deposits were stained as dark nodules. Bar = 400 μm.

Article Snippet: The concentrations of BMP2 and bFGF in the supernatants were determined with a human BMP2 ELISA kit (Boster, China) and a human bFGF ELISA kit (Boster, China), according to the manufacturers' instructions.

Techniques: Staining, Infection